Ultimate Spider-Man: Ultimate Collection

Punisher

Frank Castle

Invisible Girl

Susan Storm

Flash Thompson

bully then friend of Peter Parker

Gwen Stacy

Robbie Robertson

Kingpin

Wilson Fisk

Doctor Octopus

Otto Octavius

Venom

Eddie Brock

Black Cat

Felicia Hardy

Green Goblin

Norman Osborn

Elektra Natchios

Marvel

Harry Osborn

Green Goblin

Shadowcat

Kitty Pryde

Liz Allan

from Spider-Man, Misery

Patsy Walker

Hellcat

Electro

Max Dillon

Wong

Dr. Strange's ally

Ben Urich

Man-Thing

Ted Sallis

Lizard

Curt Connors

Clea

sorceress, Marvel

Gladiator

Kallark, Shi'ar Imperial Guard

Ben Parker

Peter Parker's uncle

Kraven the Hunter

original Sergei Kravinoff

Ben Reilly

Spider-Man / Scarlet Spider

Shocker

Herman Schultz

Boomerang

Fred Myers

Nightmare

Marvel, Fear Lord

Kong

from Spider-Man

Richard Parker

Spider-Man's Dad

Mary Parker

Spider-Man's Mom

Deathlok

Luther Manning

Samantha Hasard

Argent

Eddie Brock Jr.

Venom, typically only Earth-1610

Willie

Marvel teen-humor

Stephen Strange Jr.

Doctor Strange, (typically Ultimate only)

Mary Watson

MJ's mother, typically on Earth-1610

Eddie Brock Sr.

typically from Earth-1610