Ultimate Spider-Man

Kingpin

Wilson Fisk

Doctor Octopus

Otto Octavius

Black Cat

Felicia Hardy

Moon Knight

Marc Spector

Green Goblin

Norman Osborn

Shadowcat

Kitty Pryde

Ben Urich

Sharon Carter

Agent 13 / Iron Patriot

Ben Parker

Peter Parker's uncle

Kraven the Hunter

original Sergei Kravinoff

Henry Peter Gyrich

Marvel

Nick Fury

Earth-1610 (Ultimate)

Jimmy Woo

adversary of the Yellow Claw/S.H.I.E.L.D.

Jean DeWolff

Marvel

Richard Parker

Spider-Man's Dad

Mary Parker

Spider-Man's Mom

Mark Raxton

Molten Man

Franklin Storm

Invisible Woman and Human Torch's father

Cassandra Webb

Madame Web

Mary Watson

MJ's mother, typically on Earth-1610

Venom

Eddie Brock Jr., typically only Earth-1610

Doctor Strange

Stephen Strange Jr., (typically Ultimate only)

Eddie Brock Sr.

typically from Earth-1610

Tinkerer

Elijah Stern, typically from Earth-1610

Carnage

Gwen Stacy, typically from Earth-1610

Rhino

unknown identity from Earth-1610 (Ultimate)

Hobgoblin

Harry Osborn, typically from Earth-1610 (Ultimate Marvel)

Scorpion

unnamed clone of Peter Parker, typically from Earth-1610

Jamie Brock

mother of Eddie Brock (Venom), Marvel