Ultimate Spider-Man

Rogue

Anna Marie

J. Jonah Jameson

Carol Danvers

Ms. Marvel / Captain Marvel

Black Widow

Natasha Romanoff

Magneto

Erik Lehnsherr / Max Eisenhardt

May Parker

Peter Parker's aunt

Kitty Pryde

Sprite / Shadowcat / Star-Lord

Deadpool

Wade Wilson

Punisher

Frank Castle

Angel

Warren Worthington III

Invisible Girl

Susan Storm

Marvel Girl

Jean Grey

Spider-Woman

Jessica Drew

Flash Thompson

bully then friend of Peter Parker

Gwen Stacy

Robbie Robertson

Kingpin

Wilson Fisk

Iron Fist

Danny Rand

Doctor Octopus

Otto Octavius

Black Cat

Felicia Hardy

Moon Knight

Marc Spector

Giant-Man

Hank Pym

Green Goblin

Norman Osborn

Elektra Natchios

Marvel

Dazzler

Alison Blaire

Electro

Max Dillon

Wong

Dr. Strange's ally

Ben Urich

Shang-Chi

Master of Kung Fu

Jessica Jones

Blade

Eric Brooks

Ben Parker

Peter Parker's uncle

Karen Page

from Daredevil

Shocker

Herman Schultz

Morbius

Michael Morbius

Hammerhead

Joseph, crime boss

Nick Fury

Earth-1610 (Ultimate)

Silver Sable

Silvija Sablinova

Omega Red

Arkady Rossovich

H.E.R.B.I.E.

built by Reed Richards

Kong

from Spider-Man

Mangog

Marvel

Spot

Johnathon Ohnn

Carnage

as symbiote or unknown host

Bombshell

Lana Baumgartner, from Marvel

The Vulture

Blackie Drago

Bombshell

Lori Baumgartner

Venom

Eddie Brock Jr., typically only Earth-1610

Doctor Strange

Stephen Strange Jr., (typically Ultimate only)

Ringer

Anthony Davis

Rhino

unknown identity from Earth-1610 (Ultimate)

Hobgoblin

Harry Osborn, typically from Earth-1610 (Ultimate Marvel)

Scorpion

unnamed clone of Peter Parker, typically from Earth-1610

Mysterio

android

Beetle

Marvel, unspecified